Hcch Tocsy Assignment Of Deed

References:
A. Bax, G.M. Clore, P.C. Driscoll, A.M.Gronenborn, M. Ikura and L.E. Kay (1990) J. Magn. Reson.87 620-627. (Link to Article)
L.E. Kay, M. Ikura and A. Bax (1990) J. Am. Chem. Soc.112 888-889. (Link to Article)
M. Ikura, L.E. Kay and A. Bax (1991) J. Biomol. NMR1 299-304. (Link to Article)

Minimum labelling:15N, 13C

Dimensions: 3

Magnetisation is transferred from the side-chain hydrogen nuclei to their attached 13C nuclei. Magnetisation is then exchanged between neighbouring 13C nuclei via the J-coupling and finally transferred back to the side-chain hydrogen atoms for detection.

This spectrum can be useful in aiding side-chain assignment.

References:
A. Bax, G.M. Clore and A.M. Gronenborn (1990) J. Magn. Reson.88 425-431. (Link to Article)
E.T. Olejniczak, R.X. Xu and S.W. Fesik (1992) J. Biomol. NMR2 655-659. (Link to Article)

Minimum labelling:15N, 13C

Dimensions: 3

Magnetisation is transferred from the side-chain hydrogen nuclei to their attached 13C nuclei. This is followed by isotropic 13C mixing and finally transfer back to the side-chain hydrogen atoms for detection.

This spectrum is used for side-chain assignment. It yields strips at each carbon frequency in the side chain in which all side-chain hydrogen resonances are visible. The HCCH-COSY is a slightly less crowded version in which only the hydrogen resonances of the own and neighbouring carbons are visible. Note that the spectrum is usually displayed in such a way that the 1H detected dimension is shown along the y-axis rather than the x-axis. This means that the peaks belonging to one side-chain appear in a vertical strip rather than a horizontal strip.

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